Biological Mineralization of Methyl Orange by Pseudomonas aeruginosa

Due to its recalcitrant and carcinogenic nature, the presence of methyl orange (MO) in environment is a serious threat human animal life also toxic plants. MO being cannot be effectively reclaimed from industrial effluents through physical chemical approaches. Biological methods on other hand have potential degrade such dyes because their compatibility with nature low chances adverse effects environment. Bacteria, due fast growth rate capability surviving extreme environments can used for this purpose. In current research study, Pseudomonas aeruginosa was isolated characterized using 16rRNA textile wastewater. preliminary tests it found that has ability mineralize effectively. The physicochemical conditions were then optimized, order get maximum degradation which achieved at 37 °C, pH 7, salt concentration 0.1 g/15 mL, high carbon source 0.6 72 h experimental time. single set experiments where all these optimum combined, 88.23% decolorization selected dye achieved. At end cycle, aliquots homogenized filtered. filtrates subjected FTIR GC-MS analysis azo linkage breaking confirmed spectra. extracted ethyl acetate passed silica gel column. On basis Rf value (TLC plates used) similar fraction combined NMR analysis. compounds detected GC-MS, peaks not observed proton C-13 NMR. Instead, solvent some impurity present, showing complete mineralization had occurred action different bacterial enzymes as azoreductase, peroxidases, classes MO. prosed mechanism based spectral data needs verified by trapping individual step products use appropriate inhibitors enzymes.